genetic stability of micropropagated plantlets in date palm
نویسندگان
چکیده
randomly amplified polymorphic dna (rapd) markers were used to analyze genetic stability of the somatic embryogenesis-derived regenerants (r1-6) and mother plant in iranian date palm (phoenix dactylifera l.) cultivar khanizi. total genomic dna extracted from in vitro fresh leaves of regenerated plants and mother plant was amplified using 10-mer oligonucleotide fermantas primers. four primers of the set a to j primers were selected which revealed polymorphism and gave reproducible results. the genetic similarity between the mother plant and the callus-derived plantlets was ranged between 94% (for r1, r2) and 83% (for r5). cluster analysis by the unweighted paired group method of arithmetic mean (upgma) showed a single large cluster at an estimated similarity coefficient (90.2%).
منابع مشابه
Genetic Stability of Micropropagated Plantlets in Date Palm
Randomly amplified polymorphic DNA (RAPD) markers were used to analyze genetic stability of the somatic embryogenesis-derived regenerants (R1-6) and mother plant in Iranian date palm (Phoenix dactylifera L.) cultivar Khanizi. Total genomic DNA extracted from in vitro fresh leaves of regenerated plants and mother plant was amplified using 10-mer oligonucleotide Fermantas primers. Four primers of...
متن کاملRAPD and ISSR based evaluation of genetic stability of micropropagated plantlets of Morus alba L. variety S-1
Plant regeneration through rapid in vitro clonal propagation of nodal explants of Morus alba L. variety S-1 was established along with genetic stability analysis of regenerates. Axillary shoot bud proliferation was achieved on Murashige and Skoog (MS) medium in various culture regimes. Highest number of shoots (5.62 ± 0.01), with average length 4.19 ± 0.01 cm, was initially achieved with medium...
متن کاملAssessing Genetic Diversity of Shishi Date Palm Cultivars in Saudi Arabia and Qatar Using Microsatellite Markers
In this study fourteen microsatellite primer pairs were used to study the genetic diversity of Shishi Date palm in Qatar. A total of 32 date palm (15 Shishi cultivar, 10 Khalas and seven male date palms) were collected from Qatar and 5 Shihi cultivars were collected from Saudi Arabia for comparison. The Shishi set collected from Qatar was selected from different regions to represent the gene...
متن کاملApplication of SSR Markers for Genetic Segregation of Some Commercial Date Palm Cultivars
Cultivar identification in micro-propagated date palm seedlings is laborious so that application of molecular markers to facilitate and acceleration of the procedure seems inevitable. Given the need for control the originality of micro- propagated date palm seedlings, the aim of this study was evaluation of SSR markers usability to cultivar identification in micro-propagated date palm seedlings...
متن کاملGenetic diversity of Qatari date palm using SSR markers.
The genetic diversity in the date palm germplasm of 59 female accessions representing 12 cultivars from different locations in Qatar was investigated using 14 loci of simple-sequence repeat (SSR) primers. A total of 94 alleles, with a mean of 6.7 alleles per locus, were scored. The number of alleles per locus varied from 3 (primer mPdCIR090) to 11 (primers mPdCIR010 and mPdCIR015). The amplifie...
متن کاملGenetic characterization of Libyan date palm resources by microsatellite markers
Molecular typing of 377 female date palm trees belonging to 18 Libyan cultivars and representing common genotypes in the central Libyan oasis of Al Jufrah was performed using 16 highly polymorphic microsatellite or SSR loci. A total of 110 alleles with an average of 6.88 alleles per locus were scored indicating the high level of polymorphism existing among the cultivars thus allowing their gene...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
journal of sciences islamic republic of iranجلد ۱۶، شماره ۴، صفحات ۰-۰
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023